Fecha de publicación: 28 February 2019
Autores: Roberta Esposito, Núria Bosch, Andrés Lanzós, Taisia Polidori, Carlos Pulido-Quetglas, Rory Johnson.
Background: Long non-coding RNAs (lncRNAs) represent a huge reservoir of potential cancer targets. Such “onco-lncRNAs” have resisted traditional RNAi methods, but CRISPR-Cas9 genome editing now promises functional screens at high throughput and low cost. The unique biology of lncRNAs demands screening strategies distinct from protein-coding genes. The first such screens have identified hundreds of onco-lncRNAs promoting cell proliferation and drug resistance. Ongoing developments will further improve screen performance and translational relevance.Leer más