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Hacking the Cancer Genome: Profiling Therapeutically Actionable Long Non-coding RNAs Using CRISPR-Cas9 Screening

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Hacking the Cancer Genome: Profiling Therapeutically Actionable Long Non-coding RNAs Using CRISPR-Cas9 Screening

Cancer Cell

Fecha de publicación: 28 February 2019

DOI: https://doi.org/10.1016/j.ccell.2019.01.019

Autores: Roberta Esposito, Núria Bosch, Andrés Lanzós, Taisia Polidori, Carlos Pulido-Quetglas, Rory Johnson.

Background: Long non-coding RNAs (lncRNAs) represent a huge reservoir of potential cancer targets. Such “onco-lncRNAs” have resisted traditional RNAi methods, but CRISPR-Cas9 genome editing now promises functional screens at high throughput and low cost. The unique biology of lncRNAs demands screening strategies distinct from protein-coding genes. The first such screens have identified hundreds of onco-lncRNAs promoting cell proliferation and drug resistance. Ongoing developments will further improve screen performance and translational relevance.

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