Centrifugation‐free washing: A novel approach for removing immunoglobulin A from stored red blood cells

American Journal of Hematology 

Fecha de publicación: 29 December 2017

DOI: https://doi.org/10.1002/ajh.25026

Autores: Eszter Vörös, Nathaniel Z. Piety, Briony C. Strachan, Madeleine Lu, Sergey S. Shevkoplyas

Background: Washed red blood cells (RBCs) are indicated for immunoglobulin A (IgA) deficient recipients. Centrifugation‐based cell processors commonly used by hospital blood banks cannot consistently reduce IgA below the recommended levels, hence double washing is frequently required. Here, we describe a prototype of a simple, portable, disposable system capable of washing stored RBCs without centrifugation, while reducing IgA below 0.05 mg/dL in a single run. Samples from RBC units (n = 8, leukoreduced, 4‐6 weeks storage duration) were diluted with normal saline to a hematocrit of 10%, and then washed using either the prototype washing system, or via conventional centrifugation. 

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