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Generation of a Three-Dimensional in Vitro Ovarian Cancer Co-Culture Model for Drug Screening Assays

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Generation of a Three-Dimensional in Vitro Ovarian Cancer Co-Culture Model for Drug Screening Assays

Journal of Pharmaceutical Sciences

Fecha de publicación: 9 de abril de 2021

Autores: Larissa Bueno Tofani, Lucas Oliveira Sousa, Marcela Tavares Luiz, Juliana Palma Abriata, Juliana Maldonado Marchetti, Andréia Machado Leopoldino, Kamilla Swiech

DOI: https://doi.org/10.1016/j.xphs.2021.04.003

Background: In vitro 3D culture models have emerged in the cancer field due to their ability to recapitulate characteristics of the in vivo tumor. Herein, we described the establishment and characterization of 3D multicellular spheroids using ovarian cancer cells (SKOV-3) in co-culture with mesenchymal cells (MUC-9) or fibroblasts (CCD27-Sk). We demonstrated that SKOV-3 cells in co-culture were able to form regular and compact spheroids with diameters ranging from 300 to 400 µm and with a roundness close to 1.0 regardless of the type of stromal cell used. 

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