Fecha de publicación:
Autores: Anne C. Rios., Bianca D. Capaldo., François Vaillant., Scott N. Mueller., Geoffrey J. Lindeman., Jane E. Visvader., et al.
Background: Breast tumors are inherently heterogeneous, but the evolving cellular organization through neoplastic progression is poorly understood. Here we report a rapid, large-scale single-cell resolution 3D imaging protocol based on a one-step clearing agent that allows visualization of normal tissue architecture and entire tumors at cellular resolution. Imaging of multicolor lineage-tracing models of breast cancer targeted to either basal or luminal progenitor cells revealed profound clonal restriction during progression. Expression profiling of clones arising in Pten/Trp53-deficient tumors identified distinct molecular signatures.Leer más